AP Statistics Curriculum 2007 Contingency Indep
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===Moths Memory from Caterpillar Stage=== | ===Moths Memory from Caterpillar Stage=== | ||
- | [[AP_Statistics_Curriculum_2007_Contingency_Indep#References | One study (Blackiston, Casey and Weiss, 2008)]] showed that moths can remember something they learned at caterpillar stage -- an association of an odor with an electric shock. This memory remains even though the metamorphosis | + | [[AP_Statistics_Curriculum_2007_Contingency_Indep#References | One study (Blackiston, Casey and Weiss, 2008)]] showed that moths can remember something they learned at caterpillar stage -- an association of an odor with an electric shock. This memory remains even though the metamorphosis decomposes much of their entire (caterpillar) body and reconstitutes it again (as a moth). |
[http://en.wikipedia.org/wiki/Manduca_sexta Tobacco hornworm moth caterpillars] were put into one of 4 treatment groups: ''control'', ''shock'', ''odor'', or ''shock and odor''. Then the moths were observed to see whether they avoided the ''odor''. If the moths could "remember" the odor association with shock, then one would expect a greater rate of avoidance of odor in the moths in the last group. The empirical results seem to provide evidence in support of this hypothesis. This study employed a classical conditioning to train caterpillars to avoid the ''odor of ethyl acetate (EA)'' by pairing odor with a mild electric shock. The data are included in the table below. | [http://en.wikipedia.org/wiki/Manduca_sexta Tobacco hornworm moth caterpillars] were put into one of 4 treatment groups: ''control'', ''shock'', ''odor'', or ''shock and odor''. Then the moths were observed to see whether they avoided the ''odor''. If the moths could "remember" the odor association with shock, then one would expect a greater rate of avoidance of odor in the moths in the last group. The empirical results seem to provide evidence in support of this hypothesis. This study employed a classical conditioning to train caterpillars to avoid the ''odor of ethyl acetate (EA)'' by pairing odor with a mild electric shock. The data are included in the table below. | ||
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- | Use the SOCR Chi-square test of association to find out if indeed the data | + | Use the SOCR Chi-square test of association to find out if indeed the data support a research hypothesis that training memory may proliferate through the transition process of metamorphosis from caterpillar to moth. Also establish that the first three groups don't deviate significantly from the (null hypothesis) 50% avoidance level. Therefore, the moths seem naturally unaffected by the odor itself. You can also show that the '''Shock + Odor''' treatment group has an avoidance of the odor rate significantly higher than 0.5 (given by the null hypothesis). |
<center>[[Image:SOCR_EBook_Dinov_ChiSquare_030308_Fig4.jpg|500px]]</center> | <center>[[Image:SOCR_EBook_Dinov_ChiSquare_030308_Fig4.jpg|500px]]</center> | ||
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: ''P-value = 0.029529758678132345'' indicates that there is an association between these two variables. | : ''P-value = 0.029529758678132345'' indicates that there is an association between these two variables. | ||
- | We can also test to see if within each treatment group, there is a strong group separation between the moths that ''avoided the EA odor'' and those that ''did not''. Recall that this | + | We can also test to see if within each treatment group, there is a strong group separation between the moths that ''avoided the EA odor'' and those that ''did not''. Recall that this can be done by the [[AP_Statistics_Curriculum_2007_NonParam_2MedianPair |sign-test]]. |
: '''Controls''': <math>x_o =25</math>, and <math>P(x_o > 25) =0.230696</math>, when <math>x_o \sim Binomial(N = 46, p = 0.5)</math>. Thus, we can not reject a null hypothesis that the proportions of moths that avoided the odor and those that did not avoid the odor are different (within the '''Control group'''). | : '''Controls''': <math>x_o =25</math>, and <math>P(x_o > 25) =0.230696</math>, when <math>x_o \sim Binomial(N = 46, p = 0.5)</math>. Thus, we can not reject a null hypothesis that the proportions of moths that avoided the odor and those that did not avoid the odor are different (within the '''Control group'''). |
Revision as of 17:38, 28 June 2010
Contents |
General Advance-Placement (AP) Statistics Curriculum - Contingency Tables: Independence and Homogeneity
Contingency Tables: Independence and Homogeneity
The Chi-Square Test may also be used to assess independence and association between variables.
Motivational example
Suppose 200 randomly selected cancer patients were asked if their primary diagnosis was Brain cancer and if they owned a cell phone before their diagnosis. The results are presented in the table below.
Suppose we want to analyze the association, if any, between brain cancer and cell phone use. The 2x2 table below lists two possible outcomes for each variable (each variable is dichotomous). We have the following population parameters:
- P(CP|BC) = true probability of owning a cell phone (CP) given that the patient had brain cancer (BC). This chance may be estimated by P(CP|BC) = 0.72.
- P(CP|NBC) = true probability of owning a cell phone given that the patient had another cancer, which is estimated by P(CP|NBC) = 0.46
Brain cancer | ||||
Yes | No | Total | ||
Cell Phone Use | Yes | 18 | 80 | 98 |
No | 7 | 95 | 102 | |
Total | 25 | 175 | 200 |
Does it seem like there is an association between brain cancer and cell phone use? Of the brain cancer patients 18/25 = 0.72, owned a cell phone before their diagnosis. P(CP|BC) = 0.72, estimated probability of owning a cell phone given that the patient has brain cancer.
Of the other cancer patients, 80/175 = 0.46, owned a cell phone before their diagnosis. P(CP|NBC) = 0.46, estimated probability of owning a cell phone given that the patient has another cancer.
Calculations
- The Hypotheses:
- H_{o}: there is no association between variable 1 and variable 2 (independence)
- P(BC|CP)=P(BC), that is brain-cancer (BC) is independent of cell-phone (CP) usage.
- H_{a}: there is an association between variable 1 and variable 2 (dependence)
- Test Statistics:
The Test Statistic:
- , where df = (# rows – 1)(# columns – 1).
- Expected cell counts can be calculated by
- P-values and Critical Values for the Chi-Square Distribution may be easily computed using SOCR Distributions.
- Results:
For the brain-cancer and cell-phone usage data we have:
- P-value: and we can reject the null hypothesis at α = 0.05.
Notes
- CAUTION: Association does not imply Causality!
Contingency Tables
We now consider tables that are larger than a 2x2 (more than 2 groups or more than 2 categories), called Contingency Tables. The testing procedure is the same as the 2x2 contingency table, just more work and no possibility for a directional alternative. The goal of an contingency table is to investigate the relationship between the row and column variables
- Note: H_{o} is a compound hypothesis because it contains more than one independent assertion. This will be true for all tables larger than 2x2. In other words, the alternative hypothesis for tables larger than 2x2, will always be non-directional.
Earthquake Insurance Example
Many factors are considered when purchasing earthquake insurance. One factor of interest may be location with respect to a major earthquake fault. Suppose a survey was mailed to California residents in four counties (data shown below). Is there a statistically significant association between county of residence and purchase of earthquake insurance? Test using a = 0.05.
County | ||||||
Contra Costa (CC) | Santa Clara (SC) | Los Angeles (LA) | San Bernardino (SB) | Total | ||
Earthquake Insurance | Yes | 117 | 222 | 133 | 109 | 581 |
No | 404 | 334 | 204 | 263 | 1205 | |
Total | 521 | 556 | 337 | 372 | 1786 |
- Hypotheses:
- H_{o}: There is no association between earthquake insurance and county of residence in California. That is:
- P(Y|CC) = P(Y|SC) = P(Y|LA) = P(Y|SB)
- P(N|CC) = P(N|SC) = P(N|LA) = P(N|SB)
- H_{a}: There is an association between earthquake insurance and county of residence in California. The probability of having earthquake insurance is not the same in each county.
- P-value = 3.302154105710997E-10
Moths Memory from Caterpillar Stage
One study (Blackiston, Casey and Weiss, 2008) showed that moths can remember something they learned at caterpillar stage -- an association of an odor with an electric shock. This memory remains even though the metamorphosis decomposes much of their entire (caterpillar) body and reconstitutes it again (as a moth).
Tobacco hornworm moth caterpillars were put into one of 4 treatment groups: control, shock, odor, or shock and odor. Then the moths were observed to see whether they avoided the odor. If the moths could "remember" the odor association with shock, then one would expect a greater rate of avoidance of odor in the moths in the last group. The empirical results seem to provide evidence in support of this hypothesis. This study employed a classical conditioning to train caterpillars to avoid the odor of ethyl acetate (EA) by pairing odor with a mild electric shock. The data are included in the table below.
Group | ||||||
Control | Shock | Odor | Shock + Odor | Total | ||
Odor Avoidance | Avoided Odor | 25 | 22 | 14 | 32 | 93 |
Did not Avoid Odor | 21 | 21 | 15 | 9 | 66 | |
Total | 46 | 43 | 29 | 41 | 159 |
Use the SOCR Chi-square test of association to find out if indeed the data support a research hypothesis that training memory may proliferate through the transition process of metamorphosis from caterpillar to moth. Also establish that the first three groups don't deviate significantly from the (null hypothesis) 50% avoidance level. Therefore, the moths seem naturally unaffected by the odor itself. You can also show that the Shock + Odor treatment group has an avoidance of the odor rate significantly higher than 0.5 (given by the null hypothesis).
- Hypotheses:
- H_{o}: There is no association between odor avoidance and treatment. That is:
- P(Avoid|Shock+Odor) = P(Avoid|Odor).
- H_{a}: There is an association between odor avoidance and treatment. The probability of avoiding odor depends on the treatment applied at the larvae stage (before the metamorphosis to moths).
- P-value = 0.029529758678132345 indicates that there is an association between these two variables.
We can also test to see if within each treatment group, there is a strong group separation between the moths that avoided the EA odor and those that did not. Recall that this can be done by the sign-test.
- Controls: x_{o} = 25, and P(x_{o} > 25) = 0.230696, when . Thus, we can not reject a null hypothesis that the proportions of moths that avoided the odor and those that did not avoid the odor are different (within the Control group).
- Similarly, in the 4^{th} group, where larvae are exposed to EA odor paired with electric shock (odor prior to shock), we have: x_{o} = 32, and P(x_{o} > 32) = 0.000056, when . Thus, we can reject a null hypothesis that the proportions of moths that avoided the odor and those that did not avoid it are different (within the odor+shock treatment group). (Recall that in this non-parametric inference section we discussed these types of calculations).
Chi-Square Test Conditions
Conditions for validity of the χ^{2} test are:
- Design conditions
- for a goodness of fit, it must be reasonable to regard the data as a random sample of categorical observations from a large population.
- for a contingency table, it must be appropriate to view the data in one of the following ways:
- as two or more independent random samples, observed with respect to a categorical variable
- as one random sample, observed with respect to two categorical variables
- for either type of test, the observations within a sample must be independent of one another.
- Sample conditions
- critical values only work if each expected value > 5
Problems
References
- Blackiston DJ, Silva Casey E, Weiss MR (2008) Retention of Memory through Metamorphosis: Can a Moth Remember What It Learned As a Caterpillar? PLoS ONE 3(3): e1736. doi:10.1371/journal.pone.0001736
- SOCR Home page: http://www.socr.ucla.edu
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